SPUTUM
05/19/2021
INDICATIONS:
For the detection and characterization of premalignant/malignant pulmonary lesions or to detect infectious organisms such as Pneumocystis jiroveci. The adequacy of a sputum specimen is determined by the pres-ence of alveolar macrophages indicating that the specimen obtained is a deep cough specimen producing material from the lower airways. In addition, the specimen should not be obscured by oral or upper airway contaminants and have minimal amount of saliva present.
SPECIMEN:
5 ml (about one teaspoon) or more, if possible, of sputum obtained from a deep cough specimen.
SUPPLIES:
1. Sterile specimen cup (plastic container).
2. Specimen Requisition
3. Specimen Bag with biohazard label
COLLECTION:
When clinically feasible, sputum specimens should be obtained as follows: The optimum time for specimen collection is within 15 to 30 minutes after waking and before eating breakfast. Brushing of teeth or rinsing of the mouth thoroughly with water will reduce contamination by saliva. Instruct the patient to inhale and exhale deeply forcing air from the lungs using the diaphragm. Repeat until the patient coughs and is able to produce a sputum specimen. For patients who are unable to produce a satisfactory specimen, inhalation of a heated aerosol solution may be used to induce coughing. This technique (induced sputum) should only be performed with a trained attendant present to assist the patient. Collect the specimen in the container, attempting to obtain at least one teaspoon of sputum.Specimen should be a deep cough and not saliva. Saliva is of no diagnostic value.
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